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mouse anti gapdh  (Proteintech)


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    Structured Review

    Proteintech mouse anti gapdh
    Mouse Anti Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 10658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+gapdh/GAPDH+Monoclonal+antibody/pmc13628115-98-38-42
    Average 98 stars, based on 10658 article reviews
    mouse anti gapdh - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Membrane:

    Article Title: Acute viral infection accelerates neurodegeneration in a mouse model of ALS.
    Article Snippet: Protein was transferred onto a nitrocellulose membrane using 1X Bolt transfer buffer (ThermoFisher Scientific), containing 20% methanol, at 100 V for 60min at 4 °C. .. The membrane was blocked in 5% skim milk in Tris-buffered saline (TBS) for 1 h and then incubated with mouse anti-HA Tag (1:1000; ThermoFisher Scientific) and mouse anti-GAPDH (1:2000; ProteinTech) antibodies, diluted in 5% milk in TBS at 4 °C overnight. .. Membranes were then washed 5× with TBS with 0.05% Tween (TBS) and subsequently incubated with goat anti-mouse IRDye 800CW IgG secondary antibody (LI-COR Biosciences) diluted 1:3000 in 5% skim milk in TBS for 1 h at RT.

    Article Title: The cerebrospinal fluid-contacting nucleus CGRP-RAMP1-PKA signaling pathway mediates trigeminal neuralgia and its mechanism
    Article Snippet: The lysates were then centrifuged (15 minutes, 12,000 rpm) to collect proteins, and the concentration was measured using the BCA kit (P0012; Beyotime). .. Following 12.5% SDS-PAGE electrophoresis and polyvinylidene fluoride membrane transfer, membranes were blocked with 5% nonfat milk and incubated with the following primary antibodies: rabbit anti-CGRP (HA722991, 1:1000; HUABIO), rabbit anti-RAMP1 (ER62486, 1:1000; HUABIO), rabbit anti-p-PKA (HA721864, 1:1000; HUABIO), mouse anti-GAPDH (60004-1-Ig, 1:10000; Proteintech), or rabbit anti-β-Tubulin (80713-1-RR, 1:10000; Proteintech) to detect target proteins. ..

    Saline:

    Article Title: Acute viral infection accelerates neurodegeneration in a mouse model of ALS.
    Article Snippet: Protein was transferred onto a nitrocellulose membrane using 1X Bolt transfer buffer (ThermoFisher Scientific), containing 20% methanol, at 100 V for 60min at 4 °C. .. The membrane was blocked in 5% skim milk in Tris-buffered saline (TBS) for 1 h and then incubated with mouse anti-HA Tag (1:1000; ThermoFisher Scientific) and mouse anti-GAPDH (1:2000; ProteinTech) antibodies, diluted in 5% milk in TBS at 4 °C overnight. .. Membranes were then washed 5× with TBS with 0.05% Tween (TBS) and subsequently incubated with goat anti-mouse IRDye 800CW IgG secondary antibody (LI-COR Biosciences) diluted 1:3000 in 5% skim milk in TBS for 1 h at RT.

    Transcranial Magnetic Stimulation:

    Article Title: Acute viral infection accelerates neurodegeneration in a mouse model of ALS.
    Article Snippet: Protein was transferred onto a nitrocellulose membrane using 1X Bolt transfer buffer (ThermoFisher Scientific), containing 20% methanol, at 100 V for 60min at 4 °C. .. The membrane was blocked in 5% skim milk in Tris-buffered saline (TBS) for 1 h and then incubated with mouse anti-HA Tag (1:1000; ThermoFisher Scientific) and mouse anti-GAPDH (1:2000; ProteinTech) antibodies, diluted in 5% milk in TBS at 4 °C overnight. .. Membranes were then washed 5× with TBS with 0.05% Tween (TBS) and subsequently incubated with goat anti-mouse IRDye 800CW IgG secondary antibody (LI-COR Biosciences) diluted 1:3000 in 5% skim milk in TBS for 1 h at RT.

    Incubation:

    Article Title: Acute viral infection accelerates neurodegeneration in a mouse model of ALS.
    Article Snippet: Protein was transferred onto a nitrocellulose membrane using 1X Bolt transfer buffer (ThermoFisher Scientific), containing 20% methanol, at 100 V for 60min at 4 °C. .. The membrane was blocked in 5% skim milk in Tris-buffered saline (TBS) for 1 h and then incubated with mouse anti-HA Tag (1:1000; ThermoFisher Scientific) and mouse anti-GAPDH (1:2000; ProteinTech) antibodies, diluted in 5% milk in TBS at 4 °C overnight. .. Membranes were then washed 5× with TBS with 0.05% Tween (TBS) and subsequently incubated with goat anti-mouse IRDye 800CW IgG secondary antibody (LI-COR Biosciences) diluted 1:3000 in 5% skim milk in TBS for 1 h at RT.

    Article Title: The cerebrospinal fluid-contacting nucleus CGRP-RAMP1-PKA signaling pathway mediates trigeminal neuralgia and its mechanism
    Article Snippet: The lysates were then centrifuged (15 minutes, 12,000 rpm) to collect proteins, and the concentration was measured using the BCA kit (P0012; Beyotime). .. Following 12.5% SDS-PAGE electrophoresis and polyvinylidene fluoride membrane transfer, membranes were blocked with 5% nonfat milk and incubated with the following primary antibodies: rabbit anti-CGRP (HA722991, 1:1000; HUABIO), rabbit anti-RAMP1 (ER62486, 1:1000; HUABIO), rabbit anti-p-PKA (HA721864, 1:1000; HUABIO), mouse anti-GAPDH (60004-1-Ig, 1:10000; Proteintech), or rabbit anti-β-Tubulin (80713-1-RR, 1:10000; Proteintech) to detect target proteins. ..

    Article Title: Experimental study on ultrasound-targeted microbubble destruction promoting the proliferation, migration of human umbilical cord mesenchymal stem cells
    Article Snippet: .. The membranes were then incubated overnight at 4 °C with rabbit anti-CXCR4 (Proteintech, 10340-1-AP, 1:3000, Wuhan, Hubei Province, China) and mouse anti-GAPDH (Proteintech, 60004-1-Ig, 1:10000, Wuhan, Hubei Province, China) primary antibodies. .. After TBST washing, membranes were probed with HRP-conjugated goat anti-rabbit (Proteintech, SA00001-2, 1:10000, Wuhan, Hubei Province, China) and goat anti-mouse 14 / 50 (Proteintech, SA00001-1, 1:10000, Wuhan, Hubei Province, China) secondary antibodies for 1 hour at room temperature.

    Recombinant:

    Article Title: Boosting ovarian cancer immunotherapy via discoidin domain receptor 2 blockade and CD28 x mucin 1 bispecific antibodies
    Article Snippet: The following antibodies and reagents were used: mouse anti-collagen type I (COL1A) (#sc-59772, Santa Cruz Biotechnology, USA); anti-mouse HRP (#PAM008, SciTek Laboratories, USA); anti-human MUC1 (CD227)-PE (#130-120-142 Miltenyi Biotec, Italy); anti-human CD28-PE (#561793, BD .. AR TIC LE IN PR ES S ARTICLE IN PRESS Biosciences, Italy); mouse anti-human CD8 (#300902, Biolegend, USA); anti-human CD4-FITC (#21270043), anti-human CD3-PE (#21620034), anti-human CD8-APC (#21270086, ImmunoTools, Germany); rabbit anti-human CD3 (#AB16669, Abcam, UK); mouse anti-human CD3 clone LN10 (#PA0122, Leica Biosystems, Italy); goat anti-mouse Alexa Fluor 594 (#A11020), goat anti-rabbit Alexa Fluor 594 (#A11072), goat anti-mouse Alexa Fluor 488 (#A11070), goat anti-rabbit Alexa Fluor 488 (#A11070), goat anti-mouse Alexa Fluor 647 (#A21245), phalloidin-633 (#A22284), rabbit anti-DDR2 (#PA5-27752), 4′,6′-diamidino-2-phenykindole (DAPI) (#D1306, Thermo Fisher Scientific, Italy); rabbit anti-phospho-DDR1/DDR2 (pDDR2) (#MAB253882), anti-active human caspase-3 (#AF835, R&D Systems, USA); rat tail type 1 collagen (Col1) (#354236, Corning, USA); WRG-28 (#HY-114169, MedChemExpress, Sweden); dimethyl sulfoxide (DMSO) (#D-5879, Merck, Italy); human recombinant interleukin-2 (IL-2, #130-097-744) and IL-7 (#130-093-937, Miltenyi Biotec); rabbit anti-human granzyme B (#17215S, Cell Signalling, USA); mouse anti-GAPDH (#60004-1-IG, Proteintech, USA); CD28 x MUC1 (Tandem Fab) BsAbs (#BSFAB-H236, Creative Biolabs, USA); human Fab2 IgG (#NBP1-96779, Novus Biologicals, Bio-Techne, Italy); pembrolizumab (anti-PD1, #A2005, Selleck Biotechnology GmbH, Cologne); Cyto3D Live–Dead assay kit (#SKU:BM01, TheWell Bioscience, Inc., USA); DAPI (#1351303, Bio-Rad Laboratories, Italy); protease inhibitor MIX M (#3910201, SERVA, Germany); Mini-PROTEAN TGX Precast Protein Gels (#4561034), DC protein assay (#5000115), Clarity Western ECL Substrates (#1705061, Bio-Rad Laboratories, Italy). ..

    Live Dead Assay:

    Article Title: Boosting ovarian cancer immunotherapy via discoidin domain receptor 2 blockade and CD28 x mucin 1 bispecific antibodies
    Article Snippet: The following antibodies and reagents were used: mouse anti-collagen type I (COL1A) (#sc-59772, Santa Cruz Biotechnology, USA); anti-mouse HRP (#PAM008, SciTek Laboratories, USA); anti-human MUC1 (CD227)-PE (#130-120-142 Miltenyi Biotec, Italy); anti-human CD28-PE (#561793, BD .. AR TIC LE IN PR ES S ARTICLE IN PRESS Biosciences, Italy); mouse anti-human CD8 (#300902, Biolegend, USA); anti-human CD4-FITC (#21270043), anti-human CD3-PE (#21620034), anti-human CD8-APC (#21270086, ImmunoTools, Germany); rabbit anti-human CD3 (#AB16669, Abcam, UK); mouse anti-human CD3 clone LN10 (#PA0122, Leica Biosystems, Italy); goat anti-mouse Alexa Fluor 594 (#A11020), goat anti-rabbit Alexa Fluor 594 (#A11072), goat anti-mouse Alexa Fluor 488 (#A11070), goat anti-rabbit Alexa Fluor 488 (#A11070), goat anti-mouse Alexa Fluor 647 (#A21245), phalloidin-633 (#A22284), rabbit anti-DDR2 (#PA5-27752), 4′,6′-diamidino-2-phenykindole (DAPI) (#D1306, Thermo Fisher Scientific, Italy); rabbit anti-phospho-DDR1/DDR2 (pDDR2) (#MAB253882), anti-active human caspase-3 (#AF835, R&D Systems, USA); rat tail type 1 collagen (Col1) (#354236, Corning, USA); WRG-28 (#HY-114169, MedChemExpress, Sweden); dimethyl sulfoxide (DMSO) (#D-5879, Merck, Italy); human recombinant interleukin-2 (IL-2, #130-097-744) and IL-7 (#130-093-937, Miltenyi Biotec); rabbit anti-human granzyme B (#17215S, Cell Signalling, USA); mouse anti-GAPDH (#60004-1-IG, Proteintech, USA); CD28 x MUC1 (Tandem Fab) BsAbs (#BSFAB-H236, Creative Biolabs, USA); human Fab2 IgG (#NBP1-96779, Novus Biologicals, Bio-Techne, Italy); pembrolizumab (anti-PD1, #A2005, Selleck Biotechnology GmbH, Cologne); Cyto3D Live–Dead assay kit (#SKU:BM01, TheWell Bioscience, Inc., USA); DAPI (#1351303, Bio-Rad Laboratories, Italy); protease inhibitor MIX M (#3910201, SERVA, Germany); Mini-PROTEAN TGX Precast Protein Gels (#4561034), DC protein assay (#5000115), Clarity Western ECL Substrates (#1705061, Bio-Rad Laboratories, Italy). ..

    Protease Inhibitor:

    Article Title: Boosting ovarian cancer immunotherapy via discoidin domain receptor 2 blockade and CD28 x mucin 1 bispecific antibodies
    Article Snippet: The following antibodies and reagents were used: mouse anti-collagen type I (COL1A) (#sc-59772, Santa Cruz Biotechnology, USA); anti-mouse HRP (#PAM008, SciTek Laboratories, USA); anti-human MUC1 (CD227)-PE (#130-120-142 Miltenyi Biotec, Italy); anti-human CD28-PE (#561793, BD .. AR TIC LE IN PR ES S ARTICLE IN PRESS Biosciences, Italy); mouse anti-human CD8 (#300902, Biolegend, USA); anti-human CD4-FITC (#21270043), anti-human CD3-PE (#21620034), anti-human CD8-APC (#21270086, ImmunoTools, Germany); rabbit anti-human CD3 (#AB16669, Abcam, UK); mouse anti-human CD3 clone LN10 (#PA0122, Leica Biosystems, Italy); goat anti-mouse Alexa Fluor 594 (#A11020), goat anti-rabbit Alexa Fluor 594 (#A11072), goat anti-mouse Alexa Fluor 488 (#A11070), goat anti-rabbit Alexa Fluor 488 (#A11070), goat anti-mouse Alexa Fluor 647 (#A21245), phalloidin-633 (#A22284), rabbit anti-DDR2 (#PA5-27752), 4′,6′-diamidino-2-phenykindole (DAPI) (#D1306, Thermo Fisher Scientific, Italy); rabbit anti-phospho-DDR1/DDR2 (pDDR2) (#MAB253882), anti-active human caspase-3 (#AF835, R&D Systems, USA); rat tail type 1 collagen (Col1) (#354236, Corning, USA); WRG-28 (#HY-114169, MedChemExpress, Sweden); dimethyl sulfoxide (DMSO) (#D-5879, Merck, Italy); human recombinant interleukin-2 (IL-2, #130-097-744) and IL-7 (#130-093-937, Miltenyi Biotec); rabbit anti-human granzyme B (#17215S, Cell Signalling, USA); mouse anti-GAPDH (#60004-1-IG, Proteintech, USA); CD28 x MUC1 (Tandem Fab) BsAbs (#BSFAB-H236, Creative Biolabs, USA); human Fab2 IgG (#NBP1-96779, Novus Biologicals, Bio-Techne, Italy); pembrolizumab (anti-PD1, #A2005, Selleck Biotechnology GmbH, Cologne); Cyto3D Live–Dead assay kit (#SKU:BM01, TheWell Bioscience, Inc., USA); DAPI (#1351303, Bio-Rad Laboratories, Italy); protease inhibitor MIX M (#3910201, SERVA, Germany); Mini-PROTEAN TGX Precast Protein Gels (#4561034), DC protein assay (#5000115), Clarity Western ECL Substrates (#1705061, Bio-Rad Laboratories, Italy). ..

    DC Protein Assay:

    Article Title: Boosting ovarian cancer immunotherapy via discoidin domain receptor 2 blockade and CD28 x mucin 1 bispecific antibodies
    Article Snippet: The following antibodies and reagents were used: mouse anti-collagen type I (COL1A) (#sc-59772, Santa Cruz Biotechnology, USA); anti-mouse HRP (#PAM008, SciTek Laboratories, USA); anti-human MUC1 (CD227)-PE (#130-120-142 Miltenyi Biotec, Italy); anti-human CD28-PE (#561793, BD .. AR TIC LE IN PR ES S ARTICLE IN PRESS Biosciences, Italy); mouse anti-human CD8 (#300902, Biolegend, USA); anti-human CD4-FITC (#21270043), anti-human CD3-PE (#21620034), anti-human CD8-APC (#21270086, ImmunoTools, Germany); rabbit anti-human CD3 (#AB16669, Abcam, UK); mouse anti-human CD3 clone LN10 (#PA0122, Leica Biosystems, Italy); goat anti-mouse Alexa Fluor 594 (#A11020), goat anti-rabbit Alexa Fluor 594 (#A11072), goat anti-mouse Alexa Fluor 488 (#A11070), goat anti-rabbit Alexa Fluor 488 (#A11070), goat anti-mouse Alexa Fluor 647 (#A21245), phalloidin-633 (#A22284), rabbit anti-DDR2 (#PA5-27752), 4′,6′-diamidino-2-phenykindole (DAPI) (#D1306, Thermo Fisher Scientific, Italy); rabbit anti-phospho-DDR1/DDR2 (pDDR2) (#MAB253882), anti-active human caspase-3 (#AF835, R&D Systems, USA); rat tail type 1 collagen (Col1) (#354236, Corning, USA); WRG-28 (#HY-114169, MedChemExpress, Sweden); dimethyl sulfoxide (DMSO) (#D-5879, Merck, Italy); human recombinant interleukin-2 (IL-2, #130-097-744) and IL-7 (#130-093-937, Miltenyi Biotec); rabbit anti-human granzyme B (#17215S, Cell Signalling, USA); mouse anti-GAPDH (#60004-1-IG, Proteintech, USA); CD28 x MUC1 (Tandem Fab) BsAbs (#BSFAB-H236, Creative Biolabs, USA); human Fab2 IgG (#NBP1-96779, Novus Biologicals, Bio-Techne, Italy); pembrolizumab (anti-PD1, #A2005, Selleck Biotechnology GmbH, Cologne); Cyto3D Live–Dead assay kit (#SKU:BM01, TheWell Bioscience, Inc., USA); DAPI (#1351303, Bio-Rad Laboratories, Italy); protease inhibitor MIX M (#3910201, SERVA, Germany); Mini-PROTEAN TGX Precast Protein Gels (#4561034), DC protein assay (#5000115), Clarity Western ECL Substrates (#1705061, Bio-Rad Laboratories, Italy). ..

    Western Blot:

    Article Title: Boosting ovarian cancer immunotherapy via discoidin domain receptor 2 blockade and CD28 x mucin 1 bispecific antibodies
    Article Snippet: The following antibodies and reagents were used: mouse anti-collagen type I (COL1A) (#sc-59772, Santa Cruz Biotechnology, USA); anti-mouse HRP (#PAM008, SciTek Laboratories, USA); anti-human MUC1 (CD227)-PE (#130-120-142 Miltenyi Biotec, Italy); anti-human CD28-PE (#561793, BD .. AR TIC LE IN PR ES S ARTICLE IN PRESS Biosciences, Italy); mouse anti-human CD8 (#300902, Biolegend, USA); anti-human CD4-FITC (#21270043), anti-human CD3-PE (#21620034), anti-human CD8-APC (#21270086, ImmunoTools, Germany); rabbit anti-human CD3 (#AB16669, Abcam, UK); mouse anti-human CD3 clone LN10 (#PA0122, Leica Biosystems, Italy); goat anti-mouse Alexa Fluor 594 (#A11020), goat anti-rabbit Alexa Fluor 594 (#A11072), goat anti-mouse Alexa Fluor 488 (#A11070), goat anti-rabbit Alexa Fluor 488 (#A11070), goat anti-mouse Alexa Fluor 647 (#A21245), phalloidin-633 (#A22284), rabbit anti-DDR2 (#PA5-27752), 4′,6′-diamidino-2-phenykindole (DAPI) (#D1306, Thermo Fisher Scientific, Italy); rabbit anti-phospho-DDR1/DDR2 (pDDR2) (#MAB253882), anti-active human caspase-3 (#AF835, R&D Systems, USA); rat tail type 1 collagen (Col1) (#354236, Corning, USA); WRG-28 (#HY-114169, MedChemExpress, Sweden); dimethyl sulfoxide (DMSO) (#D-5879, Merck, Italy); human recombinant interleukin-2 (IL-2, #130-097-744) and IL-7 (#130-093-937, Miltenyi Biotec); rabbit anti-human granzyme B (#17215S, Cell Signalling, USA); mouse anti-GAPDH (#60004-1-IG, Proteintech, USA); CD28 x MUC1 (Tandem Fab) BsAbs (#BSFAB-H236, Creative Biolabs, USA); human Fab2 IgG (#NBP1-96779, Novus Biologicals, Bio-Techne, Italy); pembrolizumab (anti-PD1, #A2005, Selleck Biotechnology GmbH, Cologne); Cyto3D Live–Dead assay kit (#SKU:BM01, TheWell Bioscience, Inc., USA); DAPI (#1351303, Bio-Rad Laboratories, Italy); protease inhibitor MIX M (#3910201, SERVA, Germany); Mini-PROTEAN TGX Precast Protein Gels (#4561034), DC protein assay (#5000115), Clarity Western ECL Substrates (#1705061, Bio-Rad Laboratories, Italy). ..

    SDS Page:

    Article Title: The cerebrospinal fluid-contacting nucleus CGRP-RAMP1-PKA signaling pathway mediates trigeminal neuralgia and its mechanism
    Article Snippet: The lysates were then centrifuged (15 minutes, 12,000 rpm) to collect proteins, and the concentration was measured using the BCA kit (P0012; Beyotime). .. Following 12.5% SDS-PAGE electrophoresis and polyvinylidene fluoride membrane transfer, membranes were blocked with 5% nonfat milk and incubated with the following primary antibodies: rabbit anti-CGRP (HA722991, 1:1000; HUABIO), rabbit anti-RAMP1 (ER62486, 1:1000; HUABIO), rabbit anti-p-PKA (HA721864, 1:1000; HUABIO), mouse anti-GAPDH (60004-1-Ig, 1:10000; Proteintech), or rabbit anti-β-Tubulin (80713-1-RR, 1:10000; Proteintech) to detect target proteins. ..

    Electrophoresis:

    Article Title: The cerebrospinal fluid-contacting nucleus CGRP-RAMP1-PKA signaling pathway mediates trigeminal neuralgia and its mechanism
    Article Snippet: The lysates were then centrifuged (15 minutes, 12,000 rpm) to collect proteins, and the concentration was measured using the BCA kit (P0012; Beyotime). .. Following 12.5% SDS-PAGE electrophoresis and polyvinylidene fluoride membrane transfer, membranes were blocked with 5% nonfat milk and incubated with the following primary antibodies: rabbit anti-CGRP (HA722991, 1:1000; HUABIO), rabbit anti-RAMP1 (ER62486, 1:1000; HUABIO), rabbit anti-p-PKA (HA721864, 1:1000; HUABIO), mouse anti-GAPDH (60004-1-Ig, 1:10000; Proteintech), or rabbit anti-β-Tubulin (80713-1-RR, 1:10000; Proteintech) to detect target proteins. ..

    Incubator:

    Article Title: Experimental study on ultrasound-targeted microbubble destruction promoting the proliferation, migration of human umbilical cord mesenchymal stem cells
    Article Snippet: .. CO2 incubator (Thermo Fisher, San Jose, CA, United States); flow cytometry antibodies against CD105, CD73, CD90, CD29, CD44, CD45, CD34, CD19, CD11B, CD106, CD14, CD133, and HLA-DR (Becton, Dickinson and Company, Franklin Lakes, NJ, United States), goat serum (Boster, AR1009, Wuhan, Hubei Province, China), rabbit anti-CD90 primary antibody (Bioss, bs-0778R, Beijing, China), mouse anti-C-X-C chemokine receptor type 4 (CXCR4) primary antibody CY3 conjugated goat anti rabbit IgG antibody 8 / 50 (Boster, BA1032, Wuhan, Hubei Province, China) DAPI (Beyotime, C1002, Shanghai, China), adipogenic differentiation kit/osteogenic differentiation kit/chondrogenic differentiation kit (Wuhan Procell Biotechnology Co., Ltd., Wuhan, Hubei Province, China), Cell Counting Kit-8 (CCK-8) kit (Beyotime Biotech Inc, Shanghai, China), ultrasonic instrument (Shenzhen Suwo Medical Technology Co., Ltd., Shenzhen, Guangdong Province, China), quantitative real-time polymerase chain reaction (qPCR) system (Thermo Fisher, San Jose, CA, United States), rabbit anti-CXCR4 (Proteintech Group, Inc. Wuhan, Hubei Province, China), mouse anti-GAPDH (Proteintech Group, Inc. Wuhan, Hubei Province, China), HRP-conjugated goat anti-rabbit (Proteintech Group, Inc. Wuhan, Hubei Province, China), goat anti-mouse (Proteintech Group, Inc. Wuhan, Hubei Province, China), enhanced ECL kit (Hefei Baisha Biotechnology Co., Ltd., Anhui, Hefei Province, China) SCG-W3000 PLUS imaging system (Wuhan Seville Biotechnology Co., Ltd., Wuhan, Hubei Province, China), GraphPad Prism 10.1 software (GraphPad, La Jolla, CA, United States). ..

    Flow Cytometry:

    Article Title: Experimental study on ultrasound-targeted microbubble destruction promoting the proliferation, migration of human umbilical cord mesenchymal stem cells
    Article Snippet: .. CO2 incubator (Thermo Fisher, San Jose, CA, United States); flow cytometry antibodies against CD105, CD73, CD90, CD29, CD44, CD45, CD34, CD19, CD11B, CD106, CD14, CD133, and HLA-DR (Becton, Dickinson and Company, Franklin Lakes, NJ, United States), goat serum (Boster, AR1009, Wuhan, Hubei Province, China), rabbit anti-CD90 primary antibody (Bioss, bs-0778R, Beijing, China), mouse anti-C-X-C chemokine receptor type 4 (CXCR4) primary antibody CY3 conjugated goat anti rabbit IgG antibody 8 / 50 (Boster, BA1032, Wuhan, Hubei Province, China) DAPI (Beyotime, C1002, Shanghai, China), adipogenic differentiation kit/osteogenic differentiation kit/chondrogenic differentiation kit (Wuhan Procell Biotechnology Co., Ltd., Wuhan, Hubei Province, China), Cell Counting Kit-8 (CCK-8) kit (Beyotime Biotech Inc, Shanghai, China), ultrasonic instrument (Shenzhen Suwo Medical Technology Co., Ltd., Shenzhen, Guangdong Province, China), quantitative real-time polymerase chain reaction (qPCR) system (Thermo Fisher, San Jose, CA, United States), rabbit anti-CXCR4 (Proteintech Group, Inc. Wuhan, Hubei Province, China), mouse anti-GAPDH (Proteintech Group, Inc. Wuhan, Hubei Province, China), HRP-conjugated goat anti-rabbit (Proteintech Group, Inc. Wuhan, Hubei Province, China), goat anti-mouse (Proteintech Group, Inc. Wuhan, Hubei Province, China), enhanced ECL kit (Hefei Baisha Biotechnology Co., Ltd., Anhui, Hefei Province, China) SCG-W3000 PLUS imaging system (Wuhan Seville Biotechnology Co., Ltd., Wuhan, Hubei Province, China), GraphPad Prism 10.1 software (GraphPad, La Jolla, CA, United States). ..

    CCK-8 Assay:

    Article Title: Experimental study on ultrasound-targeted microbubble destruction promoting the proliferation, migration of human umbilical cord mesenchymal stem cells
    Article Snippet: .. CO2 incubator (Thermo Fisher, San Jose, CA, United States); flow cytometry antibodies against CD105, CD73, CD90, CD29, CD44, CD45, CD34, CD19, CD11B, CD106, CD14, CD133, and HLA-DR (Becton, Dickinson and Company, Franklin Lakes, NJ, United States), goat serum (Boster, AR1009, Wuhan, Hubei Province, China), rabbit anti-CD90 primary antibody (Bioss, bs-0778R, Beijing, China), mouse anti-C-X-C chemokine receptor type 4 (CXCR4) primary antibody CY3 conjugated goat anti rabbit IgG antibody 8 / 50 (Boster, BA1032, Wuhan, Hubei Province, China) DAPI (Beyotime, C1002, Shanghai, China), adipogenic differentiation kit/osteogenic differentiation kit/chondrogenic differentiation kit (Wuhan Procell Biotechnology Co., Ltd., Wuhan, Hubei Province, China), Cell Counting Kit-8 (CCK-8) kit (Beyotime Biotech Inc, Shanghai, China), ultrasonic instrument (Shenzhen Suwo Medical Technology Co., Ltd., Shenzhen, Guangdong Province, China), quantitative real-time polymerase chain reaction (qPCR) system (Thermo Fisher, San Jose, CA, United States), rabbit anti-CXCR4 (Proteintech Group, Inc. Wuhan, Hubei Province, China), mouse anti-GAPDH (Proteintech Group, Inc. Wuhan, Hubei Province, China), HRP-conjugated goat anti-rabbit (Proteintech Group, Inc. Wuhan, Hubei Province, China), goat anti-mouse (Proteintech Group, Inc. Wuhan, Hubei Province, China), enhanced ECL kit (Hefei Baisha Biotechnology Co., Ltd., Anhui, Hefei Province, China) SCG-W3000 PLUS imaging system (Wuhan Seville Biotechnology Co., Ltd., Wuhan, Hubei Province, China), GraphPad Prism 10.1 software (GraphPad, La Jolla, CA, United States). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Experimental study on ultrasound-targeted microbubble destruction promoting the proliferation, migration of human umbilical cord mesenchymal stem cells
    Article Snippet: .. CO2 incubator (Thermo Fisher, San Jose, CA, United States); flow cytometry antibodies against CD105, CD73, CD90, CD29, CD44, CD45, CD34, CD19, CD11B, CD106, CD14, CD133, and HLA-DR (Becton, Dickinson and Company, Franklin Lakes, NJ, United States), goat serum (Boster, AR1009, Wuhan, Hubei Province, China), rabbit anti-CD90 primary antibody (Bioss, bs-0778R, Beijing, China), mouse anti-C-X-C chemokine receptor type 4 (CXCR4) primary antibody CY3 conjugated goat anti rabbit IgG antibody 8 / 50 (Boster, BA1032, Wuhan, Hubei Province, China) DAPI (Beyotime, C1002, Shanghai, China), adipogenic differentiation kit/osteogenic differentiation kit/chondrogenic differentiation kit (Wuhan Procell Biotechnology Co., Ltd., Wuhan, Hubei Province, China), Cell Counting Kit-8 (CCK-8) kit (Beyotime Biotech Inc, Shanghai, China), ultrasonic instrument (Shenzhen Suwo Medical Technology Co., Ltd., Shenzhen, Guangdong Province, China), quantitative real-time polymerase chain reaction (qPCR) system (Thermo Fisher, San Jose, CA, United States), rabbit anti-CXCR4 (Proteintech Group, Inc. Wuhan, Hubei Province, China), mouse anti-GAPDH (Proteintech Group, Inc. Wuhan, Hubei Province, China), HRP-conjugated goat anti-rabbit (Proteintech Group, Inc. Wuhan, Hubei Province, China), goat anti-mouse (Proteintech Group, Inc. Wuhan, Hubei Province, China), enhanced ECL kit (Hefei Baisha Biotechnology Co., Ltd., Anhui, Hefei Province, China) SCG-W3000 PLUS imaging system (Wuhan Seville Biotechnology Co., Ltd., Wuhan, Hubei Province, China), GraphPad Prism 10.1 software (GraphPad, La Jolla, CA, United States). ..

    Imaging:

    Article Title: Experimental study on ultrasound-targeted microbubble destruction promoting the proliferation, migration of human umbilical cord mesenchymal stem cells
    Article Snippet: .. CO2 incubator (Thermo Fisher, San Jose, CA, United States); flow cytometry antibodies against CD105, CD73, CD90, CD29, CD44, CD45, CD34, CD19, CD11B, CD106, CD14, CD133, and HLA-DR (Becton, Dickinson and Company, Franklin Lakes, NJ, United States), goat serum (Boster, AR1009, Wuhan, Hubei Province, China), rabbit anti-CD90 primary antibody (Bioss, bs-0778R, Beijing, China), mouse anti-C-X-C chemokine receptor type 4 (CXCR4) primary antibody CY3 conjugated goat anti rabbit IgG antibody 8 / 50 (Boster, BA1032, Wuhan, Hubei Province, China) DAPI (Beyotime, C1002, Shanghai, China), adipogenic differentiation kit/osteogenic differentiation kit/chondrogenic differentiation kit (Wuhan Procell Biotechnology Co., Ltd., Wuhan, Hubei Province, China), Cell Counting Kit-8 (CCK-8) kit (Beyotime Biotech Inc, Shanghai, China), ultrasonic instrument (Shenzhen Suwo Medical Technology Co., Ltd., Shenzhen, Guangdong Province, China), quantitative real-time polymerase chain reaction (qPCR) system (Thermo Fisher, San Jose, CA, United States), rabbit anti-CXCR4 (Proteintech Group, Inc. Wuhan, Hubei Province, China), mouse anti-GAPDH (Proteintech Group, Inc. Wuhan, Hubei Province, China), HRP-conjugated goat anti-rabbit (Proteintech Group, Inc. Wuhan, Hubei Province, China), goat anti-mouse (Proteintech Group, Inc. Wuhan, Hubei Province, China), enhanced ECL kit (Hefei Baisha Biotechnology Co., Ltd., Anhui, Hefei Province, China) SCG-W3000 PLUS imaging system (Wuhan Seville Biotechnology Co., Ltd., Wuhan, Hubei Province, China), GraphPad Prism 10.1 software (GraphPad, La Jolla, CA, United States). ..

    Software:

    Article Title: Experimental study on ultrasound-targeted microbubble destruction promoting the proliferation, migration of human umbilical cord mesenchymal stem cells
    Article Snippet: .. CO2 incubator (Thermo Fisher, San Jose, CA, United States); flow cytometry antibodies against CD105, CD73, CD90, CD29, CD44, CD45, CD34, CD19, CD11B, CD106, CD14, CD133, and HLA-DR (Becton, Dickinson and Company, Franklin Lakes, NJ, United States), goat serum (Boster, AR1009, Wuhan, Hubei Province, China), rabbit anti-CD90 primary antibody (Bioss, bs-0778R, Beijing, China), mouse anti-C-X-C chemokine receptor type 4 (CXCR4) primary antibody CY3 conjugated goat anti rabbit IgG antibody 8 / 50 (Boster, BA1032, Wuhan, Hubei Province, China) DAPI (Beyotime, C1002, Shanghai, China), adipogenic differentiation kit/osteogenic differentiation kit/chondrogenic differentiation kit (Wuhan Procell Biotechnology Co., Ltd., Wuhan, Hubei Province, China), Cell Counting Kit-8 (CCK-8) kit (Beyotime Biotech Inc, Shanghai, China), ultrasonic instrument (Shenzhen Suwo Medical Technology Co., Ltd., Shenzhen, Guangdong Province, China), quantitative real-time polymerase chain reaction (qPCR) system (Thermo Fisher, San Jose, CA, United States), rabbit anti-CXCR4 (Proteintech Group, Inc. Wuhan, Hubei Province, China), mouse anti-GAPDH (Proteintech Group, Inc. Wuhan, Hubei Province, China), HRP-conjugated goat anti-rabbit (Proteintech Group, Inc. Wuhan, Hubei Province, China), goat anti-mouse (Proteintech Group, Inc. Wuhan, Hubei Province, China), enhanced ECL kit (Hefei Baisha Biotechnology Co., Ltd., Anhui, Hefei Province, China) SCG-W3000 PLUS imaging system (Wuhan Seville Biotechnology Co., Ltd., Wuhan, Hubei Province, China), GraphPad Prism 10.1 software (GraphPad, La Jolla, CA, United States). ..



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    Image Search Results


    CLE increases AMPK phosphorylation, decreases mTOR phosphorylation at Ser2448, and enhances autophagy flux in PC12 cells. (A) The Western blot (WB) analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in PC12 cells from the CTRL, MPP + , CLE, and MPP + +CLE groups. GAPDH is shown as a loading control. (B) The Quantification of p-mTOR/t-mTOR protein levels. (C) The Quantification of p-AMPK/t-AMPK protein levels. (D) The WB analysis of LC3-I and LC3-II expression. (E) The Quantification of the LC3-II/LC3-I ratio. (F) The Comparison of LC3-II/LC3-I levels in the Baf-A1, CLE, and Baf-A1+CLE groups, assessing autophagic flux. (G) The WB analysis of p62 expression. (H) The Quantification of p62 protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. CLE, clemastine; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

    Journal: Frontiers in Aging Neuroscience

    Article Title: Clemastine alleviates Parkinson’s disease pathology by activating the AMPK/mTOR pathway and enhancing autophagic flux

    doi: 10.3389/fnagi.2026.1915475

    Figure Lengend Snippet: CLE increases AMPK phosphorylation, decreases mTOR phosphorylation at Ser2448, and enhances autophagy flux in PC12 cells. (A) The Western blot (WB) analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in PC12 cells from the CTRL, MPP + , CLE, and MPP + +CLE groups. GAPDH is shown as a loading control. (B) The Quantification of p-mTOR/t-mTOR protein levels. (C) The Quantification of p-AMPK/t-AMPK protein levels. (D) The WB analysis of LC3-I and LC3-II expression. (E) The Quantification of the LC3-II/LC3-I ratio. (F) The Comparison of LC3-II/LC3-I levels in the Baf-A1, CLE, and Baf-A1+CLE groups, assessing autophagic flux. (G) The WB analysis of p62 expression. (H) The Quantification of p62 protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001; ns, not significant. CLE, clemastine; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

    Article Snippet: Proteins were transferred to PVDF membranes at 30 V for 90 min. Membranes were blocked with 5% non-fat milk in PBST for 1 h and incubated with primary antibodies for 2 h at room temperature: 1:50,000 mouse GAPDH (60004-1-Ig, Proteintech), 1:2,000 mouse p-mTOR (Ser2448) (67778-1-Ig, Proteintech), 1:5,000 mouse t-mTOR (66888-1-Ig, Proteintech), 1:1000 rabbit p-AMPK (Thr183/172) (ab133448, Abcam), 1:1000 mouse p62 (66184-1-Ig, Proteintech), 1:1,000 rabbit LC3-I/II (orb411847, Biorbyt), 1:5,000 mouse P62 (66184-1-Ig, Proteintech), 1:500 mouse α-syn (66412-1-Ig, Proteintech).

    Techniques: Phospho-proteomics, Western Blot, Analysis, Control, Expressing, Comparison

    CLE reduces α-synuclein protein levels and improves cell survival, effects inhibited by dorsomorphin (DOR). (A) Western blot (WB) and (B) The quantification of α-synuclein (α-syn) protein levels in PC12 cells from CTRL, MPP + , CLE, and MPP + +CLE groups. GAPDH is shown as a loading control. (C) Cell survival rate in CTRL, MPP + , and MPP + +CLE groups. (D, G) The WB analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in CTRL, DOR, CLE, and DOR+CLE groups. (E, F, H) The Quantification of the indicated protein ratios. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01 *** P < 0.001; ns, not significant. CLE, clemastine; α-syn, α-synuclein; CTRL, control; WB, western blot; DOR, dorsomorphin; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

    Journal: Frontiers in Aging Neuroscience

    Article Title: Clemastine alleviates Parkinson’s disease pathology by activating the AMPK/mTOR pathway and enhancing autophagic flux

    doi: 10.3389/fnagi.2026.1915475

    Figure Lengend Snippet: CLE reduces α-synuclein protein levels and improves cell survival, effects inhibited by dorsomorphin (DOR). (A) Western blot (WB) and (B) The quantification of α-synuclein (α-syn) protein levels in PC12 cells from CTRL, MPP + , CLE, and MPP + +CLE groups. GAPDH is shown as a loading control. (C) Cell survival rate in CTRL, MPP + , and MPP + +CLE groups. (D, G) The WB analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in CTRL, DOR, CLE, and DOR+CLE groups. (E, F, H) The Quantification of the indicated protein ratios. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01 *** P < 0.001; ns, not significant. CLE, clemastine; α-syn, α-synuclein; CTRL, control; WB, western blot; DOR, dorsomorphin; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

    Article Snippet: Proteins were transferred to PVDF membranes at 30 V for 90 min. Membranes were blocked with 5% non-fat milk in PBST for 1 h and incubated with primary antibodies for 2 h at room temperature: 1:50,000 mouse GAPDH (60004-1-Ig, Proteintech), 1:2,000 mouse p-mTOR (Ser2448) (67778-1-Ig, Proteintech), 1:5,000 mouse t-mTOR (66888-1-Ig, Proteintech), 1:1000 rabbit p-AMPK (Thr183/172) (ab133448, Abcam), 1:1000 mouse p62 (66184-1-Ig, Proteintech), 1:1,000 rabbit LC3-I/II (orb411847, Biorbyt), 1:5,000 mouse P62 (66184-1-Ig, Proteintech), 1:500 mouse α-syn (66412-1-Ig, Proteintech).

    Techniques: Western Blot, Control, Analysis, Comparison

    CLE increases AMPK phosphorylation, decreases mTOR phosphorylation at Ser2448, enhances autophagy, and reduces α-synuclein levels in the mouse midbrain. (A) The WB analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in the midbrain of mice from CTRL, MPTP, and MPTP+CLE groups. GAPDH is shown as a loading control. (B) The quantification of p-mTOR/t-mTOR levels. (C) The quantification of p-AMPK/t-AMPK levels. (D–F) The WB analysis of p62, LC3-I/II, and α-syn protein levels. (G–I) The quantification of p62, LC3-II/LC3-I ratio, and α-syn protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001. CLE, clemastine; α-syn, α-synuclein; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

    Journal: Frontiers in Aging Neuroscience

    Article Title: Clemastine alleviates Parkinson’s disease pathology by activating the AMPK/mTOR pathway and enhancing autophagic flux

    doi: 10.3389/fnagi.2026.1915475

    Figure Lengend Snippet: CLE increases AMPK phosphorylation, decreases mTOR phosphorylation at Ser2448, enhances autophagy, and reduces α-synuclein levels in the mouse midbrain. (A) The WB analysis of p-AMPK (Thr172), t-AMPK, p-mTOR (Ser2448), and t-mTOR protein levels in the midbrain of mice from CTRL, MPTP, and MPTP+CLE groups. GAPDH is shown as a loading control. (B) The quantification of p-mTOR/t-mTOR levels. (C) The quantification of p-AMPK/t-AMPK levels. (D–F) The WB analysis of p62, LC3-I/II, and α-syn protein levels. (G–I) The quantification of p62, LC3-II/LC3-I ratio, and α-syn protein levels. Data are presented as mean ± SEM. Tukey’s multiple comparison test was performed after one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001. CLE, clemastine; α-syn, α-synuclein; CTRL, control; WB, western blot; ANOVA, analysis of variance; SEM, standard error of the mean. n = 5 independent experiments.

    Article Snippet: Proteins were transferred to PVDF membranes at 30 V for 90 min. Membranes were blocked with 5% non-fat milk in PBST for 1 h and incubated with primary antibodies for 2 h at room temperature: 1:50,000 mouse GAPDH (60004-1-Ig, Proteintech), 1:2,000 mouse p-mTOR (Ser2448) (67778-1-Ig, Proteintech), 1:5,000 mouse t-mTOR (66888-1-Ig, Proteintech), 1:1000 rabbit p-AMPK (Thr183/172) (ab133448, Abcam), 1:1000 mouse p62 (66184-1-Ig, Proteintech), 1:1,000 rabbit LC3-I/II (orb411847, Biorbyt), 1:5,000 mouse P62 (66184-1-Ig, Proteintech), 1:500 mouse α-syn (66412-1-Ig, Proteintech).

    Techniques: Phospho-proteomics, Analysis, Control, Comparison, Western Blot